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NAD+ Reconstitution: How Much Water and What Units

Published August 1, 2026 · Educational, not medical advice

NAD+ usually ships as a freeze-dried powder that you reconstitute before use. This page shows how the water you add sets the concentration, how to read the units to draw, and how to store a mixed vial — with a free calculator to do the arithmetic. It is mixing-math, not a dose recommendation; your dose and route are decisions for your clinician.

The math

Concentration = vial mg × 1000 ÷ water mL (in mcg/mL). Units = dose ÷ concentration × 100 on a U-100 syringe. Remember 1 mg = 1000 mcg.

Worked example

Illustrative only, not a recommendation. A 500 mg NAD+ vial with 5 mL of bacteriostatic water is 100 mg/mL. For a 50 mg amount: 50 ÷ 100 = 0.5 mL = 50 units. NAD+ vials are often larger (500–1000 mg), so a bit more water keeps the draw and any injection-site volume comfortable.

Diluent and storage

NAD+ is water-soluble and is commonly reconstituted with bacteriostatic water. Once mixed, keep it refrigerated and protected from light, and don’t freeze the solution. NAD+ is relatively sensitive, so use it within its window and discard if it discolors or clouds.

NAD+ is often started low because a fast push can feel uncomfortable — but how much, how fast, and by what route are medical decisions for your clinician, not something a calculator decides.

Run your numbers

Enter your vial size, the water you added, and your amount in the free calculator to see the exact units for a clean draw.

Do the math yourself — free
Enter your vial, your water, and your dose; the calculator shows the exact units to draw. It never recommends a dose.
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Common questions

How much bac water do I add to an NAD+ vial?

There is no single right amount — more water is more dilute (a larger draw), less is more concentrated. Many people pick a volume that gives a clean, easy-to-read unit count; the calculator solves for that.

What concentration do I get?

Concentration = vial mg times 1000, divided by the water in mL (in mcg/mL). A 500 mg vial in 5 mL is 100 mg/mL.

How do I store reconstituted NAD+?

Keep it refrigerated, protected from light, and do not freeze the solution. Use within its window and discard if it clouds or discolors.

Is this a dose recommendation?

No. It is mixing-math only. Your dose and route are decisions for your clinician.

How do I read the units on the syringe?

On a U-100 insulin syringe, 100 units = 1 mL. The units to draw are your amount divided by the concentration, times 100.

Related reading

Sources

Do this math automatically. Pepathary works out the units to draw, then logs the dose and tracks the vial — offline, on your phone. Get the app.
Pepathary is a personal organizational and educational tool. It is not a medical device, does not diagnose, treat, cure, or prevent any condition, and does not recommend medications or doses. Figures here summarize widely-reported information and general guidance; always confirm what is right for you with your physician or pharmacist.

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